HIMA F7553

Description

HIMA F7553Upon receipt the kit should be stored at 4°C if intended for use within
24 hours. Otherwise the Assay Plate, Standard, Detection Reagent A,
and Detection Reagent B should be stored at -20°C. Avoid repeated
freeze-thaw cycles. Store all other kit components at 4°C. The
Substrate should never be frozen. Once individual reagents are opened
it is recommended that the kit be used within 1 month. Unused Strip
Plate wells should be stored at -20°C in a sealed bag containing
desiccant in order to minimize exposure to moisture. Do not use the kit
beyond its expiration date.
OTHER REQUIRED SUPPLIES
 Microplate reader with 450nm wavelength filter
 High-precision pipette and sterile pipette tips
 Eppendorf tubes
 37°C incubator
 Deionized or distilled water
 Absorbent paper
The following is an example of how to layout a study. A dilution series
of the positive control Standard should be run in duplicate or triplicate,
with the last well in each series being the negative control blank.
Samples should also be run in duplicate or triplicate. Unknown samples
should be run as a dilution series in order to identify the optimal
dilution that produces an OD reading within the OD range of the
positive control Standard dilution series.

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